Dataset

Neuropeptides

Neuropeptides, including Substance P, Beta-endorphin, Dynorphin A, neurotensin, and Leu-enkaphalin, measured with traditional enzymatic tests, such as Enzyme-Linked Immunosorbent Assays (ELISA).

Urinary substance P (YC_ACTIONBB1_1, YC_ACTIONBB1_2, YC_ACTIONBB2, YC_ACTIONBB3), Beta-endorphin (YC_ACTIONBB1_1, YC_ACTIONBB1_2), Dynorphin A (YC_ACTIONBB1_1, YC_ACTIONBB1_2), neurotensin (YC_ACTIONBB1_2, YC_ACTIONBB1_2), and Leu-enkaphalin (YC_ACTIONBB1_1, YC_ACTIONBB1_2) were measured using ELISA. For more information see: Appendix 2 dissertation Fiona Hagenbeek (https://research.vu.nl/en/publications/omics-biomarkers-and-aggressive-behavior) and https://doi.org/10.3389/fpsyt.2020.00165

Labels	biological sample biomarkers neuropeptides urine neurotransmitters ELISA laboratory
Constructs	physiology
Available measurements	Young Netherlands Twin Register YC_ACTIONBB1_1 C 10.0 - 13.0 years From 2014-01-01 to 2015-01-01 YC_ACTIONBB1_2 C 10.0 - 13.0 years From 2014-01-01 to 2015-01-01 YC_ACTIONBB2 C 6.0 - 11.0 years From 2015-01-01 to 2015-01-01 YC_ACTIONBB3 C 6.0 - 13.0 years From 2016-01-01 to 2017-01-01
Mode of collection	MeasurementsAndTests Biological sample/measurement

You can also access this dataset using the API (see API Docs).

author	None
author_email	None
creator_user_id	6c404c5c-dbec-4933-bc54-0b7656c773ba
dc_additional_info	Urinary substance P (YC_ACTIONBB1_1, YC_ACTIONBB1_2, YC_ACTIONBB2, YC_ACTIONBB3), Beta-endorphin (YC_ACTIONBB1_1, YC_ACTIONBB1_2), Dynorphin A (YC_ACTIONBB1_1, YC_ACTIONBB1_2), neurotensin (YC_ACTIONBB1_2, YC_ACTIONBB1_2), and Leu-enkaphalin (YC_ACTIONBB1_1, YC_ACTIONBB1_2) were measured using ELISA. For more information see: Appendix 2 dissertation Fiona Hagenbeek (https://research.vu.nl/en/publications/omics-biomarkers-and-aggressive-behavior) and https://doi.org/10.3389/fpsyt.2020.00165
dc_analysis_unit	[]
dc_cohort	NTR
dc_mode_of_collection	MeasurementsAndTests
dc_mode_of_collection_sub	Biological sample/measurement
id	91cdd6f5-dde0-43c7-aedf-3bf0065fa229
isopen	False
license_title	None
maintainer	None
maintainer_email	None
metadata_created	2023-03-28T10:01:13.525329
metadata_modified	2023-04-04T12:48:54.559880
name	fbedc8666e7042066be96359b0fbecff
notes	Neuropeptides, including Substance P, Beta-endorphin, Dynorphin A, neurotensin, and Leu-enkaphalin, measured with traditional enzymatic tests, such as Enzyme-Linked Immunosorbent Assays (ELISA).
num_resources	0
num_tags	0
organization	{'id': '1c79e3cf-de04-4f4c-aa77-46aff3228db4', 'name': 'ntr', 'title': 'NTR', 'type': 'organization', 'description': 'The Netherlands Twin Register (NTR) consists of individuals who have agreed to participate in twin-family studies and was established and is managed by the Department of Biological Psychology, VU University Amsterdam (see: https://tweelingenregister.vu.nl/). The NTR was established in 1987 and has enrolled around 120.000 twins and a roughly equal number of their relatives. The majority of twin families have participated one or more times in survey studies on a host of epidemiological variables including social demography, personality, lifestyle, somatic and mental health, and subsamples took part in biomaterial collection (e.g. DNA) and dedicated projects for e.g. neuropsychological, biomarker, and behavioural traits. The recruitment into the NTR is fully inclusive without any restrictions on enrolment. The NTR is committed to actively seek the commitment of its participants to science and their continued involvement in NTR research by maintaining a website, electronic newsletters, and the MijnNTR portal that provides personalized feedback to participants. The NTR resources, e.g. the longitudinal phenotyping, the extended pedigree structures, and the multi-generation genotyping allow for future twin-family research that will contribute to gene discovery, causality modelling, and studies of genetic and cultural inheritance of behavioural traits and mental and physical health. In the CD2 portal, only the NTR projects for children and adolescents (<18 years of age) have been included. This is refered to as the Young Netherlands Twin Register (YNTR).', 'image_url': 'https://beta.data.individualdevelopment.nl/img/logo_ntr.png', 'created': '2023-03-06T15:49:42.299075', 'is_organization': True, 'approval_status': 'approved', 'state': 'active'}
owner_org	1c79e3cf-de04-4f4c-aa77-46aff3228db4
private	False
state	active
title	Neuropeptides
type	dataset
dc_constructs	[{'dc_construct': 'physiology'}]
dc_labels	[{'dc_label': 'biological sample'}, {'dc_label': 'biomarkers'}, {'dc_label': 'neuropeptides'}, {'dc_label': 'urine'}, {'dc_label': 'neurotransmitters'}, {'dc_label': 'ELISA'}, {'dc_label': 'laboratory'}]
measure_age_range	[{'measure_age_month': 72}, {'measure_age_month': 73}, {'measure_age_month': 74}, {'measure_age_month': 75}, {'measure_age_month': 76}, {'measure_age_month': 77}, {'measure_age_month': 78}, {'measure_age_month': 79}, {'measure_age_month': 80}, {'measure_age_month': 81}, {'measure_age_month': 82}, {'measure_age_month': 83}, {'measure_age_month': 84}, {'measure_age_month': 85}, {'measure_age_month': 86}, {'measure_age_month': 87}, {'measure_age_month': 88}, {'measure_age_month': 89}, {'measure_age_month': 90}, {'measure_age_month': 91}, {'measure_age_month': 92}, {'measure_age_month': 93}, {'measure_age_month': 94}, {'measure_age_month': 95}, {'measure_age_month': 96}, {'measure_age_month': 97}, {'measure_age_month': 98}, {'measure_age_month': 99}, {'measure_age_month': 100}, {'measure_age_month': 101}, {'measure_age_month': 102}, {'measure_age_month': 103}, {'measure_age_month': 104}, {'measure_age_month': 105}, {'measure_age_month': 106}, {'measure_age_month': 107}, {'measure_age_month': 108}, {'measure_age_month': 109}, {'measure_age_month': 110}, {'measure_age_month': 111}, {'measure_age_month': 112}, {'measure_age_month': 113}, {'measure_age_month': 114}, {'measure_age_month': 115}, {'measure_age_month': 116}, {'measure_age_month': 117}, {'measure_age_month': 118}, {'measure_age_month': 119}, {'measure_age_month': 120}, {'measure_age_month': 121}, {'measure_age_month': 122}, {'measure_age_month': 123}, {'measure_age_month': 124}, {'measure_age_month': 125}, {'measure_age_month': 126}, {'measure_age_month': 127}, {'measure_age_month': 128}, {'measure_age_month': 129}, {'measure_age_month': 130}, {'measure_age_month': 131}, {'measure_age_month': 132}, {'measure_age_month': 133}, {'measure_age_month': 134}, {'measure_age_month': 135}, {'measure_age_month': 136}, {'measure_age_month': 137}, {'measure_age_month': 138}, {'measure_age_month': 139}, {'measure_age_month': 140}, {'measure_age_month': 141}, {'measure_age_month': 142}, {'measure_age_month': 143}, {'measure_age_month': 144}, {'measure_age_month': 145}, {'measure_age_month': 146}, {'measure_age_month': 147}, {'measure_age_month': 148}, {'measure_age_month': 149}, {'measure_age_month': 150}, {'measure_age_month': 151}, {'measure_age_month': 152}, {'measure_age_month': 153}, {'measure_age_month': 154}, {'measure_age_month': 155}, {'measure_age_month': 156}]
wave	[{'wave_ckan_id': '493fa4aa87b127de5472cfc648ada5f1', 'wave_cohort': 'NTR: Young Netherlands Twin Register', 'wave_cohort_ckan_id': '6f88a94b0bf79a8304ba604c655c08a9', 'wave_description': 'This is the first wave of the technical pilot of the ACTION project (Aggression in Children: Unraveling gene-environment interplay to inform Treatment and interventION strategies Consortium; http://www.action-euproject.eu/). The aim of the technical pilot was to test the collection protocols for urine and buccal sample collection in children. The technical pilot comprised of twin participants with a low aggression score (T-score<65) on the mother-reported ASEBA CBCL Aggression Syndrome Scale at age 3 and/or age 7. Urine samples with minimal information regarding urine collection were collected at two timepoints with an interval of approximately two weeks. Urine samples contributed to the optimization of the biomarker assays and the liquid and gas chromatography mass spectrometry protocols for analyzing urine samples in children. In the technical pilot a urine dipstick and assessment of urine density (specific gravity) were obtained in addition to 15 biomarker assays (creatinine, neopterin, C-peptide, Substance P, Oxidized DNA/RNA, Procalcitonin, total antioxidant capacity, glucose, Thiobarbituric acid reactive species, beta-endorphin, cholecytokin, leu-enkaphalin, dynorphin A, albumin, and neurotensin) , and two metabolomics platforms (liquid chromatography mass spectrometry platform targeting amines and gas chromatography mass spectrometry platform targeting organic acids). Buccal-cell samples were also collected at both time points where samples obtained from wave 1 were genotyped on the Affymetrix Axiom genotyping array. ', 'wave_end_date_collection': '2015-01-01', 'wave_start_date_collection': '2014-01-01', 'wave_subject_codes': ['C'], 'wave_title': 'YC_ACTIONBB1_1', 'wave_universe': '120-156'}, {'wave_ckan_id': '2e5c638578137ebadfe308ae32addd35', 'wave_cohort': 'NTR: Young Netherlands Twin Register', 'wave_cohort_ckan_id': '6f88a94b0bf79a8304ba604c655c08a9', 'wave_description': 'This is the second wave of the technical pilot of the ACTION project (Aggression in Children: Unraveling gene-environment interplay to inform Treatment and interventION strategies Consortium; http://www.action-euproject.eu/). The aim of the technical pilot was to test the collection protocols for urine and buccal sample collection in children. The technical pilot comprised of twin participants with a low aggression score (T-score<65) on the mother-reported ASEBA CBCL Aggression Syndrome Scale at age 3 and/or age 7. Urine samples with minimal information regarding urine collection were collected at two timepoints with an interval of approximately two weeks. Urine samples contributed to the optimization of the biomarker assays and the liquid and gas chromatography mass spectrometry protocols for analyzing urine samples in children. In the technical pilot a urine dipstick and assessment of urine density (specific gravity) were obtained in addition to 15 biomarker assays (creatinine, neopterin, C-peptide, Substance P, Oxidized DNA/RNA, Procalcitonin, total antioxidant capacity, glucose, Thiobarbituric acid reactive species, beta-endorphin, cholecytokin, leu-enkaphalin, dynorphin A, albumin, and neurotensin) , and two metabolomics platforms (liquid chromatography mass spectrometry platform targeting amines and gas chromatography mass spectrometry platform targeting organic acids). Buccal-cell samples were also collected at both time points where samples obtained from wave 1 were genotyped on the Affymetrix Axiom genotyping array. ', 'wave_end_date_collection': '2015-01-01', 'wave_start_date_collection': '2014-01-01', 'wave_subject_codes': ['C'], 'wave_title': 'YC_ACTIONBB1_2', 'wave_universe': '120-156'}, {'wave_ckan_id': '2698643e5b9a7a2b266f22734b0967aa', 'wave_cohort': 'NTR: Young Netherlands Twin Register', 'wave_cohort_ckan_id': '6f88a94b0bf79a8304ba604c655c08a9', 'wave_description': 'This is the Biochemical Study of the ACTION project (Aggression in Children: Unraveling gene-environment interplay to inform Treatment and interventION strategies Consortium; http://www.action-euproject.eu/). The aim of the biochemical study was to further validate the biomarker assays and metabolomics platforms selected during the technical pilot in a larger twin sample. The twins were selected based on their mother-rated aggression scores (Achenbach System of Empirically Based Assessment (ASEBA) Child Behavior Checklist) at age 3 and/or 7. The Biochemical study comprised concordant low-scoring twin pairs, concordant high-scoring twin pairs, and a discordant low-high scoring twin pairs. High aggression was defined as having an ASEBA CBCL Aggression Syndrome Scale T-score equal to or larger than 65, and low aggression as an ASEBA CBCL Aggression Syndrome Scale T-score of less than 65. Here, data collection comprised of first-morning urine samples and buccal-cell DNA samples in the twins and family members living at the same address as the participating twin pairs were also asked to provide a buccal-cell DNA sample. During the urine and buccal-cell collection, parents of the twins were asked to complete the ASEBA CBCL for school-aged children (6-18 years) to reflect the current child behaviors, and to provide information about health condition of the twins and conditions of urine collection. Biochemical measurement in urine included a dipstick, density (specific gravity), creatinine, neopterin, C-peptide, Substance P, oxidized DNA/RNA, procalcitonin, total antioxidant capacity, and glucose, and two metabolomics platforms: 1) a liquid chromatography mass spectrometry (LC-MS) platform targeting amines; and 2) a LC-MS platform targeting steroid hormones. Buccal-cell samples genotyped on the Affymetrix Axiom genotyping array. ', 'wave_end_date_collection': '2015-01-01', 'wave_start_date_collection': '2015-01-01', 'wave_subject_codes': ['C'], 'wave_title': 'YC_ACTIONBB2', 'wave_universe': '72-132'}, {'wave_ckan_id': '2e0143a5f2a71b8c14d7a4e47ef791bb', 'wave_cohort': 'NTR: Young Netherlands Twin Register', 'wave_cohort_ckan_id': '6f88a94b0bf79a8304ba604c655c08a9', 'wave_description': 'This is the Main Study of the ACTION project (Aggression in Children: Unraveling gene-environment interplay to inform Treatment and interventION strategies Consortium; http://www.action-euproject.eu/). Here, data collection of first-morning urine samples for metabolomics biomarker discovery and buccal-cell DNA samples for (epi)genetics was conducted in a large cohort of young twins. Family members living at the same address as the participating twin pairs were also asked to provide a buccal-cell DNA sample. The twins were selected to be part of a concordant low-scoring twin pair, a concordant high-scoring twin pair, or a discordant twin pair. Aggression had been assessed in the past by the age-appropriate version of the CBCL (Achenbach System of Empirically Based Assessment (ASEBA) Child Behavior Checklist). During the urine and buccal-cell collection, parents of the twins were asked to complete the ASEBA CBCL for school-aged children (6-18 years) to reflect the current child behaviors, and to provide information about health condition of the twins and conditions of urine collection. Biochemical measurement in urine included a dipstick, density (specific gravity), creatinine, neopterin, C-peptide, Substance P, and oxidized DNA/RNA. Metabolomics data of the Main Study together with the samples obtained in the Biochemical Study (ACTIONBB2) were were obtained from three metabolomics platforms: 1) a liquid chromatography mass spectrometry (LC-MS) platform targeting amines; 2) a LC-MS platform targeting steroid hormones; and 3) a gas chromatography (GC) MS platform targeting organic acids. Buccal-cell samples of the twins from the Main Study together with the samples obtained in the Biochemical Study (ACTIONBB2) were used for genome-wide methylation measurement on the Infinium MethylationEPIC BeadChip Kit (after conclussion of the ACTION project additional twin pairs and siblings were also measured on this platform). DNA samples from all family members were genotyped on the Affymetrix Axiom or Illumina GSA genotyping arrays.', 'wave_end_date_collection': '2017-01-01', 'wave_start_date_collection': '2016-01-01', 'wave_subject_codes': ['C'], 'wave_title': 'YC_ACTIONBB3', 'wave_universe': '72-156'}]
resources	[]
tags	[]
groups	[]
relationships_as_subject	[]
relationships_as_object	[]
tracking_summary	{'total': 29, 'recent': 3}